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双水相萃取法分离鱼腥草总黄酮的研究
Alternative TitleOptimization of aqueous two-phase extraction of total flavonoids from houttuyniae cordata thunb.
杨靖1; 吕瑞2; 邓俊华2
Corresponding Author吕瑞
2016-12
Source Publication食品工业科技
ISSN1002-0306
Volume37Issue:24Pages:319-323
Other Abstract

 利用聚乙二醇(PEG)-硫酸铵双水相体系对鱼腥草总黄酮进行萃取分离。通过单因素试验分别考察了PEG分子量、两相质量比、萃取温度、萃取p H对分离提取鱼腥草中总黄酮的影响,并用正交试验优化了萃取工艺。经优化后,鱼腥草总黄酮在以m PEG800:m(NH4)2SO4=2.0:2.0为双水相体系,萃取p H为3.0,水浴温度为50℃时,萃取率可高达98.96%。本方法简单高效,绿色环保,可为鱼腥草总黄酮的高效利用提供参考。 
 

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To separate the total flavonoids from Houttuyniae cordata Thunb by aueous two-phase
systems composed of polyethylene glycol (PEG) and phosphate salts, an orthogonal experimental design method was applied to evaluate the influence of the average molecular mass of PEG, mass ratio of the two phases, temperature and pH value on total flavonoids extraction. The extraction rate was achieved 98.96 % under the optimal conditions: mPEG400: m(NH4)2SO4=2.0: 2.0, temperature was 50 ℃, pH was 3.0. The result indicated that, the aqueous two-phase extraction was simple, high quality and environment friendly, and also can apply some useful information for the efficientuse of the total flavonoids in Houttuyniae cordata Thunb.

Keyword双水相萃取 鱼腥草 总黄酮 正交实验法
Subject AreaTs201.1
Indexed ByCSCD ; 北大中文核心
Language中文
CSCD IDCSCD:5874340
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/18133
Collection管理与支撑系统
综合测试与模拟中心
Affiliation1.中科院科学院成都山地灾害与环境研究所
2.绵阳师范学院化学与化学工程学院
First Author Affilication中国科学院水利部成都山地灾害与环境研究所
Recommended Citation
GB/T 7714
杨靖,吕瑞,邓俊华. 双水相萃取法分离鱼腥草总黄酮的研究[J]. 食品工业科技,2016,37(24):319-323.
APA 杨靖,吕瑞,&邓俊华.(2016).双水相萃取法分离鱼腥草总黄酮的研究.食品工业科技,37(24),319-323.
MLA 杨靖,et al."双水相萃取法分离鱼腥草总黄酮的研究".食品工业科技 37.24(2016):319-323.
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