|Alternative Title||Soil Characteristics and Lonicera japonica Introduced in the Haihuogou area|
|Place of Conferral||北京|
|Keyword||金银花 引种 生态适应性 Issr Its|
In China, honeysuckle (Lonicera japonica Thumb.) is one of the traditional Chinese medicine. In this study, collected 20 mainstream cultivars in Shangdong, Henan and Hebei and 1 wild breed in Sichuan, by comparing the differences of soil properties and climate in provenance and study area, observing the phenophase of blooming, testing flower buds growth index, yield and content of medicinal composition, measured the ecological adaptability of introduced honeysuckle in Hailuogou area. By comparing ITS sequence and analysis of ISSR genetic diversity, the study also evaluated and identified the germplasm, analyzed the genetic background and relationship of introduced honeysuckle. The target of the study is finding some kind of honeysuckles with high-yield, high-quality, strong adaptability and genetic stability planted in Hailuogou area. The main results were as follows:(1) The climate and soil adaptability of the introduced honeysuckle in Hailuogou areaClimate in Hailuogou area is different in the main producing areas. The Hailuogou have small annual variation of temperatures, rainfall is much and light is shorter. The soil in Hailuogou area is acidic and pH is slightly lower than that of the main producing areas. CEC content is higher than that of the main producing areas. C, N, P contents and storages is adequate. Content of K, closely related to growth of honeysuckle, is slightly different with the main producing areas, the content of Ca is slightly lower, and Mn, Fe, P, Cu and Zn is slightly higher. Ni and Cd exceed the secondary standard of environmental quality standard for soil. After a year of experiment, the honeysuckle was growing and flowering normally in Hailuogou area.(2) Select two varieties of honeysuckle J13 and J21 are suitable for cultivation in Hailuogou area.With the difference in varieties and ecological adaptability of introduced L. japonica, the flowering time, bud development, yield and the content chlorogenic acid and cynaroside are different. According to flower bud growth and yield index and combining with difference of chlorogenic acid and cynaroside content, J13 and J21is fit for planting in Hailuogou area, with high yield and quality.(3) Genetic analysis of introduced varietiesAnalysis of ITS sequence alignment and ISSR genetic diversity divide introduced varieties into honeysuckle (Lonicera japonica Thunb.) and flos lonicerae (Lonicera macranthoides Hand.-Mazz.). The J13 and J21 are clustered in different subgroups of L. japonica group in cluster analysis, which the kinds of differences between the two species can be stableily inherited in their respective product lines, clear for two different varieties.
|何海燕. 海螺沟土壤特性与金银花选种[D]. 北京. 中国科学院大学,2016.|
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