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The microbial gene diversity along an elevation gradient of the Tibetan grassland
Yang, Yunfeng1; Gao, Ying1; Wang, Shiping2; Xu, Depeng1; Yu, Hao3; Wu, Linwei1; Lin, Qiaoyan4; Hu, Yigang4,5; Li, Xiangzhen6; He, Zhili3; Deng, Ye3; Zhou, Jizhong1,3,7
2014-02-01
Source PublicationISME JOURNAL
ISSN1751-7362
Volume8Issue:2Pages:430-440
AbstractTibet is one of the most threatened regions by climate warming, thus understanding how its microbial communities function may be of high importance for predicting microbial responses to climate changes. Here, we report a study to profile soil microbial structural genes, which infers functional roles of microbial communities, along four sites/elevations of a Tibetan mountainous grassland, aiming to explore the potential microbial responses to climate changes via a strategy of space-for-time substitution. Using a microarray-based metagenomics tool named GeoChip 4.0, we showed that microbial communities were distinct for most but not all of the sites. Substantial variations were apparent in stress, N and C-cycling genes, but they were in line with the functional roles of these genes. Cold shock genes were more abundant at higher elevations. Also, gdh converting ammonium into urea was more abundant at higher elevations, whereas ureC converting urea into ammonium was less abundant, which was consistent with soil ammonium contents. Significant correlations were observed between N-cycling genes (ureC, gdh and amoA) and nitrous oxide flux, suggesting that they contributed to community metabolism. Lastly, we found by Canonical correspondence analysis, Mantel tests and the similarity tests that soil pH, temperature, NH4+-N and vegetation diversity accounted for the majority (81.4%) of microbial community variations, suggesting that these four attributes were major factors affecting soil microbial communities. On the basis of these observations, we predict that climate changes in the Tibetan grasslands are very likely to change soil microbial community functional structure, with particular impacts on microbial N-cycling genes and consequently microbe-mediated soil N dynamics.
Keywordgene diversity soil microbial community community metabolism alpine grassland elevation gradient
DOI10.1038/ismej.2013.146
WOS KeywordECOSYSTEM CO2 EXCHANGE ; GEOCHIP-BASED ANALYSIS ; ALPINE MEADOW ; BACTERIAL COMMUNITIES ; SOIL-MOISTURE ; PLATEAU ; SEA ; PATTERNS ; NITROGEN ; DESERT
Language英语
Funding ProjectNational Science Foundation of China[41171201] ; National High Technology Research and Development Program of China[2012AA061401] ; National Basic Research Program[2010CB833502] ; United States Department of Energy, Biological Systems Research on the Role of Microbial Communities in C Cycling Program[DE-SC0004601] ; Oklahoma Bioenergy Center (OBC) ; ENIGMA-Ecosystems and Networks Integrated with Genes and Molecular Assemblies through the Office of Science, Office of Biological and Environmental Research, of the US Department of Energy[DE-AC02-05CH11231] ; United States Department of Agriculture through NSF-USDA Microbial Observatories Program[2007-35319-18305]
WOS Research AreaEnvironmental Sciences & Ecology ; Microbiology
WOS SubjectEcology ; Microbiology
WOS IDWOS:000330386500016
Funding OrganizationNational Science Foundation of China ; National High Technology Research and Development Program of China ; National Basic Research Program ; United States Department of Energy, Biological Systems Research on the Role of Microbial Communities in C Cycling Program ; Oklahoma Bioenergy Center (OBC) ; ENIGMA-Ecosystems and Networks Integrated with Genes and Molecular Assemblies through the Office of Science, Office of Biological and Environmental Research, of the US Department of Energy ; United States Department of Agriculture through NSF-USDA Microbial Observatories Program
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:101[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/22747
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorYang, Yunfeng
Affiliation1.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Inst Tibetan Plateau Res, Lab Alpine Ecol & Biodivers, Beijing, Peoples R China
3.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adapt & Evolut Plateau Biota, Xining, Peoples R China
5.Chinese Acad Sci, Cold & Arid Reg & Environm & Engn Res Inst, Shapotou Desert Expt & Res Stn, Lanzhou, Peoples R China
6.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China
7.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
Recommended Citation
GB/T 7714
Yang, Yunfeng,Gao, Ying,Wang, Shiping,et al. The microbial gene diversity along an elevation gradient of the Tibetan grassland[J]. ISME JOURNAL,2014,8(2):430-440.
APA Yang, Yunfeng.,Gao, Ying.,Wang, Shiping.,Xu, Depeng.,Yu, Hao.,...&Zhou, Jizhong.(2014).The microbial gene diversity along an elevation gradient of the Tibetan grassland.ISME JOURNAL,8(2),430-440.
MLA Yang, Yunfeng,et al."The microbial gene diversity along an elevation gradient of the Tibetan grassland".ISME JOURNAL 8.2(2014):430-440.
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