IMHE OpenIR  > 山地表生过程与生态调控重点实验室
Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol)
Cheng, Jian-Hua1,2; Tang, Xiang-Yu1; Cui, Jun-Fang1
2019-01-10
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
EISSN1879-1026
Volume647Pages:853-861
SubtypeArticle
AbstractThe application of animal manure is a highly recommended traditional agricultural practice for soils of relatively low fertility. However, for the farmland purple soils that are widely distributed in the upper Yangtze River region, little knowledge has been established in previous studies about the changes in the antibiotic resistome upon manure amendment. In the present study, the impact of long-term pig manure slurry application on the occurrence of antibiotic resistance genes (ARGs) and bacterial community was assessed in arable calcareous purple soil using high-throughput quantitative polymerase chain reaction and Illumina sequencing. Four treatments, including a non-fertilization control (CK) and pig manure (OM), OM plus mineral N fertilizer (OMN) and OM plus mineral NPK fertilizer (OMNPK) treatments were investigated. Across all the soil samples receiving different treatments, a total of 139 unique ARGs and 6 mobile genetic element genes were detected, with multidrug and beta-lactam the two most dominant types of ARGs. The results of the principal coordinate analysis (PCoA) suggest that the profiles of soil ARGs in the two treatments of OM combined with mineral fertilizer(s) (i.e., OMN and OMNPK) were similar to those in the control treatment, while the soil receiving only pig manure application had a different pattern of ARGs from the soils in the other three treatments. A clear reduction of soil ARGs was observed in the OM treatment. Significant and positive relationships were found not only among ARGs but also between mobile genetic elements (MGEs) and ARGs. However, no significant relationships were detected between ARG patterns and bacterial community composition. These results imply that the long-term application of pig manure slurry to purple soil does not lead to the prevalence of ARGs; however, the potential for the horizontal transfer of ARGs in calcareous purple soil should not be ignored. (C) 2018 Elsevier B.V. All rights reserved.
KeywordLong-term manure amendment Antibiotic resistance genes Horizontal gene transfer Co-occurrence Purple soil
DOI10.1016/j.scitotenv.2018.08.028
Indexed BySCI
WOS KeywordRECLAIMED WATER IRRIGATION ; AGRICULTURAL SOILS ; TETRACYCLINE RESISTANCE ; ANTIMICROBIAL RESISTANCE ; BACTERIAL-RESISTANCE ; CHEMICAL FERTILIZERS ; EASTERN CHINA ; HEAVY-METALS ; SWINE MANURE ; ABUNDANCE
Language英语
Quartile2区
Funding ProjectNational Key Research and Development Program of China[2016YFD0800203] ; National Natural Science Foundation of China[41790431] ; National Natural Science Foundation of China[41471268] ; CAS Key Research Program of Frontier Sciences[QYZDJ-SSW-DQC006] ; 135 Strategic Program of the Institute of Mountain Hazards and Environment, CAS[SDS-135-1702]
TOP
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000447090400086
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; 135 Strategic Program of the Institute of Mountain Hazards and Environment, CAS
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/24041
Collection山地表生过程与生态调控重点实验室
Corresponding AuthorTang, Xiang-Yu
Affiliation1.Chinese Acad Sci, Inst Mt Hazards & Environm, Key Lab Mt Surface Proc & Ecol Regulat, Chengdu 610041, Sichuan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author Affilication中国科学院水利部成都山地灾害与环境研究所
Corresponding Author Affilication中国科学院水利部成都山地灾害与环境研究所
Recommended Citation
GB/T 7714
Cheng, Jian-Hua,Tang, Xiang-Yu,Cui, Jun-Fang. Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,647:853-861.
APA Cheng, Jian-Hua,Tang, Xiang-Yu,&Cui, Jun-Fang.(2019).Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol).SCIENCE OF THE TOTAL ENVIRONMENT,647,853-861.
MLA Cheng, Jian-Hua,et al."Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol)".SCIENCE OF THE TOTAL ENVIRONMENT 647(2019):853-861.
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