IMHE OpenIR
Inhibition of microRNA-129-5p expression ameliorates ultraviolet ray-induced corneal epithelial cell injury via upregulation of EGFR
Yang, Ying1,2; Gong, Bo2,3; Wu, Zheng-Zheng1,2; Shuai, Ping2,4; Li, Dong-Feng1,2; Liu, Ling-Lin1,2; Yu, Man1,2
Corresponding AuthorYu, Man(yuman712@126.com)
2019-07-01
Source PublicationJOURNAL OF CELLULAR PHYSIOLOGY
ISSN0021-9541
Volume234Issue:7Pages:11692-11707
AbstractExisting evidence has highlighted the effect of ultraviolet light radiation leading to corneal epithelium impairment. During this study, we aim to investigate the effect of microRNA-129-5p (miR-129-5p) on the wound healing process of corneal epithelial cells (CECs) induced by ultraviolet rays in mice by targeting epidermal growth factor receptor (EGFR). First, mouse models of ultraviolet ray-induced CEC injury were established and intrastromally injected with different mimic, inhibitor, and short interfering RNA (siRNA) to detect the effect of miR-129-5p on CEC injury. Subsequently, the corneal tissues were obtained to detect the antioxidant ability and EGFR-positive expression rate. The dual-luciferase reporter gene assay was used to test whether EGFR could directly target miR-129-5p. To further investigate the specific mechanism of miR-129-5p and EGFR in CEC injury, CECs were cultured and transfected with miR-129-5p mimic, miR-129-5p inhibitor, siRNA-EGFR, and miR-129-5p inhibitor+siRNA-EGFR. miR-129-5p has been proven to directly target EGFR. Inhibition of miR-129-5p is able to increase the antioxidant capacity, EGFR-positive rate and the expressions of EGFR, B-cell lymphoma-2, zonula occluden-1, occludin, and keratinocyte growth factor-2, but decrease the expression of vascular endothelial growth factor, BCL2-associated X protein, interleukin (IL)-1, and IL-4. Inhibition of miR-129-5p arrests cells at the S and G2 phases and decreases apoptosis. Our study provides evidence stating that inhibiting miR-129-5p and upregulating EGFR could aid in the repair of mice CEC injury induced by ultraviolet radiation. Therefore, inhibition of miR-129-5p might provide a basic theory in the repair of CEC injury caused by ultraviolet rays.
Keywordcorneal epithelial cells EGFR microRNA-129-5 ultraviolet rays wound healing
DOI10.1002/jcp.27837
Indexed BySCI
WOS KeywordSIGNALING PATHWAY ; CARCINOMA-CELLS ; GROWTH ; PROLIFERATION ; MIR-129-5P ; APOPTOSIS ; MIGRATION ; CANCER
Language英语
Funding ProjectBasic Research Fund of Provincial Public-Benefit Scientific Institute of Sichuan Academy of Medical Sciences[2018YSKY0017-14]
WOS Research AreaCell Biology ; Physiology
WOS SubjectCell Biology ; Physiology
WOS IDWOS:000462645700164
Funding OrganizationBasic Research Fund of Provincial Public-Benefit Scientific Institute of Sichuan Academy of Medical Sciences
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/25891
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorYu, Man
Affiliation1.Sichuan Acad Med Sci, Dept Ophthalmol, 32,West Sect 2,First Ring Rd, Chengdu 610072, Sichuan, Peoples R China
2.Chinese Acad Sci, Sichuan Prov Peoples Hosp, Sichuan Translat Med Res Hosp, Chengdu, Sichuan, Peoples R China
3.Sichuan Acad Med Sci, Key Lab Human Dis Gene Study Sichuan Prov, Chengdu, Sichuan, Peoples R China
4.Sichuan Acad Med Sci, Hlth Management Ctr, Chengdu, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Yang, Ying,Gong, Bo,Wu, Zheng-Zheng,et al. Inhibition of microRNA-129-5p expression ameliorates ultraviolet ray-induced corneal epithelial cell injury via upregulation of EGFR[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(7):11692-11707.
APA Yang, Ying.,Gong, Bo.,Wu, Zheng-Zheng.,Shuai, Ping.,Li, Dong-Feng.,...&Yu, Man.(2019).Inhibition of microRNA-129-5p expression ameliorates ultraviolet ray-induced corneal epithelial cell injury via upregulation of EGFR.JOURNAL OF CELLULAR PHYSIOLOGY,234(7),11692-11707.
MLA Yang, Ying,et al."Inhibition of microRNA-129-5p expression ameliorates ultraviolet ray-induced corneal epithelial cell injury via upregulation of EGFR".JOURNAL OF CELLULAR PHYSIOLOGY 234.7(2019):11692-11707.
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