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Metabolomics reveals the key role of oxygen metabolism in heat susceptibility of an alpine-dwelling ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)
Zhu, Wei1,2; Meng, Qian1; Zhang, Huan1; Wang, Meng-Long1; Li, Xuan1; Wang, Hong-Tuo1; Zhou, Gui-Ling1; Miao, Lin1; Qin, Qi-Lian1; Zhang, Ji-Hong1
Corresponding AuthorQin, Qi-Lian(qinql@ioz.ac.cn) ; Zhang, Ji-Hong(zhangjh@ioz.ac.cn)
2019-08-01
Source PublicationINSECT SCIENCE
ISSN1672-9609
Volume26Issue:4Pages:695-710
AbstractGhost moths inhabiting the alpine meadows of the Tibetan Plateau are cold-adapted stenothermal organisms that are susceptible to heat (dead within 7 days at 27 degrees C exposure). Exploring the metabolic basis of their heat susceptibility would extend our understanding of the thermal biology of alpine-dwelling invertebrates. Here, gas chromatography-mass spectrometry-based metabolomics was combined with physiological and transcriptional approaches to determine the metabolic mechanisms of heat susceptibility in Thitarodes xiaojinensis larvae. The metabolomics results showed that 27 degrees C heat stress impaired the Krebs cycle and lipolysis in T. xiaojinensis larvae, as demonstrated by the accumulation of intermediary metabolites. In addition, carbohydrate reserves were highly and exclusively consumed, and an anaerobic product, lactate, accumulated. This evidence suggested a strong reliance on glycolysis to anaerobically generate energy. The respiration rate and enzymatic activity test results indicated a deficiency in O-2 metabolism; in addition, the Krebs cycle capacity was not decreased, and the metabolic flux through aerobic pathways was limited. These findings were further supported by the occurrence of hypoxia symptoms in midgut mitochondria (vacuolation and swelling) and increased transcription of hypoxia-induced factor 1-alpha. Overall, heat stress caused O-2 limitation and depressed the overall intensity of aerobic metabolism in ghost moths, and less efficient anaerobic glycolysis was activated to sustain their energy supply. As carbohydrates were depleted, the energy supply became deficient. Our study presents a comprehensive metabolic explanation for the heat susceptibility of ghost moths and reveals the relationship between O-2 metabolism and heat susceptibility in these larvae.
Keywordenergy metabolism ghost moths metabolomics thermal intolerance oxygen limitation respiration rate
DOI10.1111/1744-7917.12605
Indexed BySCI
WOS KeywordLIMITED THERMAL TOLERANCE ; TEMPERATURE-DEPENDENT BIOGEOGRAPHY ; HIGH-ALTITUDE ADAPTATION ; COD GADUS-MORHUA ; BINDING PROPERTIES ; CLIMATE-CHANGE ; LIMITATION ; HEMOGLOBIN ; STRESS ; INSECT
Language英语
Funding ProjectNational Nature Science Foundation of China[31272366] ; National Nature Science Foundation of China[31772525] ; National High Technology Research and Development Program (863 Program) of China[2011AA10A204] ; State Key Laboratory of Integrated Management of Pest Insects and Rodents[IPM 1504]
WOS Research AreaEntomology
WOS SubjectEntomology
WOS IDWOS:000465438800009
Funding OrganizationNational Nature Science Foundation of China ; National High Technology Research and Development Program (863 Program) of China ; State Key Laboratory of Integrated Management of Pest Insects and Rodents
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26143
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorQin, Qi-Lian; Zhang, Ji-Hong
Affiliation1.Chinese Acad Sci, State Key Lab Integrated Management Pest Insects, Inst Zool, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Chengdu Inst Biol, Dept Herpetol, Chengdu, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Wei,Meng, Qian,Zhang, Huan,et al. Metabolomics reveals the key role of oxygen metabolism in heat susceptibility of an alpine-dwelling ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)[J]. INSECT SCIENCE,2019,26(4):695-710.
APA Zhu, Wei.,Meng, Qian.,Zhang, Huan.,Wang, Meng-Long.,Li, Xuan.,...&Zhang, Ji-Hong.(2019).Metabolomics reveals the key role of oxygen metabolism in heat susceptibility of an alpine-dwelling ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae).INSECT SCIENCE,26(4),695-710.
MLA Zhu, Wei,et al."Metabolomics reveals the key role of oxygen metabolism in heat susceptibility of an alpine-dwelling ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)".INSECT SCIENCE 26.4(2019):695-710.
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