IMHE OpenIR
Functional study of the association of CHI3L1 polymorphisms with asthma susceptibility in the Southwest Chinese Han population
Chen, Guo1,2,3,4; Zhang, Miao-Miao1; Wang, Yu1; Wu, Shou-Quan1; Wang, Ming-Gui1; He, Jian-Qing1
Corresponding AuthorHe, Jian-Qing(jianqhe@gmail.com)
2019-05-14
Source PublicationBIOSCIENCE REPORTS
ISSN0144-8463
Volume39Pages:10
AbstractBackground: Chitinase 3-like 1 (CHI3L1) is involved in the Th2 cell mediated pathway, tissue remodeling and fibrosis. Correlations of CHI3L1 gene polymorphisms with asthma in previous studies have been inconsistent. The present study was designed to investigate the association between CHI3L1 polymorphisms and asthma in the southwest Chinese Han population. Methods: Two single nucleotide polymorphisms (SNPs), rs4950928 and rs10399931, were genotyped in 410 asthma patients and 418 healthy controls from Southwest China. Dual-luciferase reporter gene analysis was performed to detect allele-dependent promoter activity of CHI3L1 variants in HEK293 cells. Real-time quantitative PCR was applied to detect the relative mRNA expression associated with different genotypes of CHI3L1 rs10399931. A meta-analysis was performed using data collected from previously published reports and the present study. Results: No significant association was found between rs4950928 and asthma. The rs10399931 CT/TT genotype increased the risk of asthma under the dominant model (P = 0.031, OR = 1.428, 95% CI, 1.033-1.974), while the CT genotype showed the same tendency under the heterozygous model (P = 0.003, OR = 1.680, 95% CI, 1.186-2.380). No statistically significant difference was found between alleles T and C of rs10399931 in the dual-luciferase reporter gene analysis (P = 0.201). The rs10399931 CT/TT genotypes reduced the relative mRNA expression detected by real-time quantitative PCR (P = 0.002). There was no significant association between the CHI3L1 rs4950928 polymorphism and the risk of asthma in the meta-analysis. Conclusion: In the southwest Chinese Han population, the CHI3L1 rs10399931 CT/TT genotypes may increase the risk of asthma. rs10399931 may be a functional variant of CHI3L1 due to its effect on mRNA expression.
DOI10.1042/BSR20182008
Indexed BySCI
WOS KeywordCHITINASE 3-LIKE 1 ; YKL-40 LEVELS ; SERUM YKL-40 ; GENETIC-VARIATION ; PROMOTER ; CHILDREN
Language英语
Funding ProjectNational Natural Science Foundation of China[81370121] ; Health and Family Planning Commission of Sichuan Province Project[16PJ413] ; Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Project[2017QN11] ; Sichuan Provincial Cadre Health Research Project[2018-211]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000469169900001
Funding OrganizationNational Natural Science Foundation of China ; Health and Family Planning Commission of Sichuan Province Project ; Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Project ; Sichuan Provincial Cadre Health Research Project
PublisherPORTLAND PRESS LTD
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Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26412
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorHe, Jian-Qing
Affiliation1.Sichuan Univ, West China Hosp, Dept Resp & Crit Care Med, Chengdu, Sichuan, Peoples R China
2.Sichuan Acad Med Sci, Dept Geriatr, Chengdu, Sichuan, Peoples R China
3.Sichuan Prov Peoples Hosp, Chengdu, Sichuan, Peoples R China
4.Chinese Acad Sci, Sichuan Translat Med Res Hosp, Chengdu, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Guo,Zhang, Miao-Miao,Wang, Yu,et al. Functional study of the association of CHI3L1 polymorphisms with asthma susceptibility in the Southwest Chinese Han population[J]. BIOSCIENCE REPORTS,2019,39:10.
APA Chen, Guo,Zhang, Miao-Miao,Wang, Yu,Wu, Shou-Quan,Wang, Ming-Gui,&He, Jian-Qing.(2019).Functional study of the association of CHI3L1 polymorphisms with asthma susceptibility in the Southwest Chinese Han population.BIOSCIENCE REPORTS,39,10.
MLA Chen, Guo,et al."Functional study of the association of CHI3L1 polymorphisms with asthma susceptibility in the Southwest Chinese Han population".BIOSCIENCE REPORTS 39(2019):10.
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