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Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
Fang Xianping1,2; Xin Ya3; Sheng Zheliang4; Liu Hui3; Jiang Aili2; Wang Fang5; Yang Jian1; Xi Xiaojun2; Zha Qian2; Zhang Liqing2; Dai Liangying6; Yan Chengqi5; Chen Jianping1
2018
Source PublicationJournal of Agricultural and Food Chemistry
ISSN0021-8561
EISSN1520-5118
Volume66Issue:50Pages:13310-13320
SubtypeArticle
Contribution Rank4
AbstractThe various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition. Copyright © 2018 American Chemical Society.
KeywordAmino acids Fruits Metabolism Molecular biology Plants (botany)
DOI10.1021/acs.jafc.8b02708
Indexed ByEI
Language英语
EI Accession NumberAccession number:20185206283213
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26621
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorYan Chengqi
Affiliation1.Institute of Plant Virology, Ningbo University, Ningbo;315211, China;
2.Institute of Forestry and Pomology, Shanghai Academy of Agricultural Sciences, Shanghai;201403, China;
3.Hangzhou Academy of Agricultural Sciences, Hangzhou;310024, China;
4.Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu;610041, China;
5.Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo;315040, China;
6.College of Plant Protection, Hunan Agricultural University, Changsha;410128, China
Recommended Citation
GB/T 7714
Fang Xianping,Xin Ya,Sheng Zheliang,et al. Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata[J]. Journal of Agricultural and Food Chemistry,2018,66(50):13310-13320.
APA Fang Xianping.,Xin Ya.,Sheng Zheliang.,Liu Hui.,Jiang Aili.,...&Chen Jianping.(2018).Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata.Journal of Agricultural and Food Chemistry,66(50),13310-13320.
MLA Fang Xianping,et al."Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata".Journal of Agricultural and Food Chemistry 66.50(2018):13310-13320.
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