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Adenosine Monophosphate - Activated Protein Kinase Signaling Regulates Lipid Metabolism in Response to Salinity Stress in the Red-Eared Slider Turtle Trachemys scripta elegans
Hong, Meiling1; Li, Na1; Li, Jiangyue1; Li, Weihao1; Liang, Lingyue1; Li, Qian1; Wang, Runqi1; Shi, Haitao1,2; Storey, Kenneth B.3; Ding, Li1
Corresponding AuthorDing, Li(dingli@hainnu.edu.cn)
2019-07-31
Source PublicationFRONTIERS IN PHYSIOLOGY
ISSN1664-042X
Volume10Pages:11
AbstractAquatic animals have developed various mechanisms to live in either hyperionic or hypoionic environments, and, as such, not many species are capable of surviving in both. The red-eared slider turtle, Trachemys scripta elegans, a well-known freshwater species, has recently been found to invade and inhabit brackish water. Herein, we focus on some of the metabolic adaptations that are required to survive and cope with salinity stress. The regulation of the adenosine monophosphate (AMP)-activated protein kinase (AMPK), a main cellular "energy sensor," and its influence on lipid metabolism were evaluated with a comparison of three groups of turtles: controls in freshwater, and turtles held in water of either 5 parts per thousand salinity (S5) or 15 parts per thousand salinity (S15) with sampling at 6, 24, and 48 h and 30 days of exposure. When subjected to elevated salinities of 5 or 15 parts per thousand, AMPK mRNA levels and AMPK enzyme activity increased strongly. In addition, the high expression of the peroxisome proliferator activated receptor-alpha (PPAR alpha) transcription factor that, in turn, facilitated upregulation of target genes including carnitine palmitoyltransferase (CPT) and acyl-CoA oxidase (ACO). Furthermore, the expression of transcription factors involved in lipid synthesis such as the carbohydrate-responsive element-binding protein (ChREBP) and sterol regulatory element-binding protein 1c (SREBP-1c) was inhibited, and two of their target genes, acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), were significantly decreased. Moreover, exposure to saline environments also increased plasma triglyceride (TG) content. Interestingly, the content of low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) in plasma was markedly higher than the control in the S15 group after 30 days, which indicated that lipid metabolism was disrupted by chronic exposure to high salinity. These findings demonstrate that activation of AMPK might regulate lipid metabolism in response to salinity stress through the inhibition of lipid synthesis and promotion of lipid oxidation in the liver of T s. elegans. This may be an important component of the observed salinity tolerance of these turtles that allow for invasion of brackish waters.
KeywordTrachemys scripta elegans salinity adaptation fatty acid oxidation lipid synthesis lipid metabolism-related transcription factors
DOI10.3389/fphys.2019.00962
Indexed BySCI
WOS KeywordELEMENT-BINDING PROTEIN ; CHAR SALVELINUS-ALPINUS ; GENE-EXPRESSION ; SUBUNIT ISOFORM ; AMPK ; GLUCOSE ; TRANSCRIPTION ; INSULIN ; GROWTH ; PHOSPHORYLATION
Language英语
Funding ProjectNational Natural Science Foundation of China[31760116] ; National Natural Science Foundation of China[31772486] ; Hainan Natural Science Foundation[2019CXTD404] ; Hainan Natural Science Foundation[318MS046] ; Hainan Science Research Project of Higher Education[Hnky2016-15] ; Hainan Innovation Research Project of Graduate[Hys2018-234]
WOS Research AreaPhysiology
WOS SubjectPhysiology
WOS IDWOS:000478029400001
Funding OrganizationNational Natural Science Foundation of China ; Hainan Natural Science Foundation ; Hainan Science Research Project of Higher Education ; Hainan Innovation Research Project of Graduate
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26833
Collection中国科学院水利部成都山地灾害与环境研究所
Corresponding AuthorDing, Li
Affiliation1.Hainan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Ecol Trop Isl, Haikou, Hainan, Peoples R China
2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China
3.Carleton Univ, Dept Biol, Ottawa, ON, Canada
Recommended Citation
GB/T 7714
Hong, Meiling,Li, Na,Li, Jiangyue,et al. Adenosine Monophosphate - Activated Protein Kinase Signaling Regulates Lipid Metabolism in Response to Salinity Stress in the Red-Eared Slider Turtle Trachemys scripta elegans[J]. FRONTIERS IN PHYSIOLOGY,2019,10:11.
APA Hong, Meiling.,Li, Na.,Li, Jiangyue.,Li, Weihao.,Liang, Lingyue.,...&Ding, Li.(2019).Adenosine Monophosphate - Activated Protein Kinase Signaling Regulates Lipid Metabolism in Response to Salinity Stress in the Red-Eared Slider Turtle Trachemys scripta elegans.FRONTIERS IN PHYSIOLOGY,10,11.
MLA Hong, Meiling,et al."Adenosine Monophosphate - Activated Protein Kinase Signaling Regulates Lipid Metabolism in Response to Salinity Stress in the Red-Eared Slider Turtle Trachemys scripta elegans".FRONTIERS IN PHYSIOLOGY 10(2019):11.
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