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Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds
He Haiyan1,2; Zhang Dan1; Gao Jianing1,2; Andersen Theis Raaschou3; Mou Zishen4,5
2019
Source PublicationPEERJ
ISSN2167-8359
Volume7Pages:e7636
SubtypeArticle
Contribution Rank1
AbstractLonicera japonica flos (LJF), the dried flower buds of L. japonica Thunb., have been used in traditional Chinese herbal medicine for thousands of years. Recent studies have reported that LJF has many medicinal properties because of its antioxidative, hypoglycemic, hypolipidemic, anti-allergic, anti-inflammatory, and antibacterial effects. LJF is widely used in China in foods and healthcare products, and is contained in more than 30% of current traditional Chinese medicine prescriptions. Because of this, many Chinese villages cultivate LJF instead of traditional crops due to its high commercial value in the herbal medicine market. Since 2005, the flower buds of L. japonica are the only original LW parts considered according to the Chinese Pharmacopoeia of the People's Republic of China. However, for historical and commercial reasons, some closely related species of Lonicera Linn. continue to be mislabeled and used as LJF. Currently, there are hundreds of commercial varieties of LW on the market and it is difficult to choose fine LJF varieties to cultivate. In this study, a total of 21 varieties labeled as LJF on the market were planted in the Hailuogou area. In order to choose the optimum variety, internal transcribed spacer (ITS) sequence alignment analysis was used to test whether the 21 varieties were genuine LJF or not. Cluster analysis of active components based on the content of chlorogenic acid and luteoloside in flower buds, stems and leaves was used to evaluate the quality of the varieties. Results demonstrated that four of the varieties were L. macranthoides Hand.-Mazz., while the other 17 varieties were L. japonica, and genuine LJF. The ITS sequence analysis was proven to be highly effective in identifying LJF and Lonicerae flos. Among the 17 L. japonica varieties, the amounts of chlorogenic acid and luteoloside in flower buds, stems and leaves were significantly different. Based on the cluster analysis method, the variety H11 was observed to have the highest level of active components, and is therefore recommended for large-scale planting in the Hailuogou area.
KeywordLonicera japonica Thumb. ITS sequence Identification Chlorogenic acid Luteoloside Evaluation
DOI10.7717/peerj.7636
Indexed BySCI
Language英语
WOS IDWOS:000483511700006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26956
Collection山地表生过程与生态调控重点实验室
Corresponding AuthorZhang Dan
Affiliation1.Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, People's Republic of China;
2.University of Chinese Academy of Sciences, Beijing, People's Republic of China;
3.Centre for Applied Research & Development, VIA University College, Horsens, Denmark;
4.State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution (Chengdu University of Technology), Chengdu, People's Republic of China;
5.State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu, People's Republic of China
First Author Affilication中国科学院水利部成都山地灾害与环境研究所
Corresponding Author Affilication中国科学院水利部成都山地灾害与环境研究所
Recommended Citation
GB/T 7714
He Haiyan,Zhang Dan,Gao Jianing,et al. Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds[J]. PEERJ,2019,7:e7636.
APA He Haiyan,Zhang Dan,Gao Jianing,Andersen Theis Raaschou,&Mou Zishen.(2019).Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds.PEERJ,7,e7636.
MLA He Haiyan,et al."Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds".PEERJ 7(2019):e7636.
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