| Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds | |
He, Haiyan1,2; Zhang, Dan1 ; Gao, Jianing1,2; Andersen, Theis Raaschou3; Mou, Zishen4,5
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| Corresponding Author | Zhang, Dan(daniezhang@imde.ac.cn) |
| 2019-09-03 | |
| Source Publication | PEERJ
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| ISSN | 2167-8359 |
| Volume | 7Pages:17 |
| Abstract | Lonicera japonica flos (LJF), the dried flower buds of L. japonica Thunb., have been used in traditional Chinese herbal medicine for thousands of years. Recent studies have reported that LJF has many medicinal properties because of its antioxidative, hypoglycemic, hypolipidemic, anti-allergic, anti-inflammatory, and antibacterial effects. LJF is widely used in China in foods and healthcare products, and is contained in more than 30% of current traditional Chinese medicine prescriptions. Because of this, many Chinese villages cultivate LJF instead of traditional crops due to its high commercial value in the herbal medicine market. Since 2005, the flower buds of L. japonica are the only original LW parts considered according to the Chinese Pharmacopoeia of the People's Republic of China. However, for historical and commercial reasons, some closely related species of Lonicera Linn. continue to be mislabeled and used as LJF. Currently, there are hundreds of commercial varieties of LW on the market and it is difficult to choose fine LJF varieties to cultivate. In this study, a total of 21 varieties labeled as LJF on the market were planted in the Hailuogou area. In order to choose the optimum variety, internal transcribed spacer (ITS) sequence alignment analysis was used to test whether the 21 varieties were genuine LJF or not. Cluster analysis of active components based on the content of chlorogenic acid and luteoloside in flower buds, stems and leaves was used to evaluate the quality of the varieties. Results demonstrated that four of the varieties were L. macranthoides Hand.-Mazz., while the other 17 varieties were L. japonica, and genuine LJF. The ITS sequence analysis was proven to be highly effective in identifying LJF and Lonicerae flos. Among the 17 L. japonica varieties, the amounts of chlorogenic acid and luteoloside in flower buds, stems and leaves were significantly different. Based on the cluster analysis method, the variety H11 was observed to have the highest level of active components, and is therefore recommended for large-scale planting in the Hailuogou area. |
| Keyword | Lonicera japonica Thumb. ITS sequence Identification Chlorogenic acid Luteoloside Evaluation |
| DOI | 10.7717/peerj.7636 |
| Indexed By | SCI |
| WOS Keyword | NUCLEAR RIBOSOMAL DNA ; FLOWER BUDS ; PCR-RFLP ; THUNB. ; ANTIOXIDANT ; ACIDS ; EXTRACTION ; COMPONENTS ; REGION ; POLYSACCHARIDE |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41571315] ; CAS President's International Fellowship Initiative (PIFI)[2018VCB0018] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000483511700006 |
| Funding Organization | National Natural Science Foundation of China ; CAS President's International Fellowship Initiative (PIFI) |
| Publisher | PEERJ INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imde.ac.cn/handle/131551/26956 |
| Collection | 山地表生过程与生态调控重点实验室 |
| Corresponding Author | Zhang, Dan |
| Affiliation | 1.Chinese Acad Sci, Inst Mt Hazards & Environm, Key Lab Mt Surface Proc & Ecol Regulat, Chengdu, Sichuan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.VIA Univ Coll, Ctr Appl Res & Dev, Horsens, Denmark 4.Chengdu Univ Technol, State Environm Protect Key Lab Synerget Control &, Chengdu, Sichuan, Peoples R China 5.State Key Lab Geohazard Prevent & Geoenvironm Pro, Chengdu, Sichuan, Peoples R China |
| First Author Affilication | 中国科学院水利部成都山地灾害与环境研究所 |
| Corresponding Author Affilication | 中国科学院水利部成都山地灾害与环境研究所 |
| Recommended Citation GB/T 7714 | He, Haiyan,Zhang, Dan,Gao, Jianing,et al. Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds[J]. PEERJ,2019,7:17. |
| APA | He, Haiyan,Zhang, Dan,Gao, Jianing,Andersen, Theis Raaschou,&Mou, Zishen.(2019).Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds.PEERJ,7,17. |
| MLA | He, Haiyan,et al."Identification and evaluation of Lonicera japonica flos introduced to the Hailuogou area based on ITS sequences and active compounds".PEERJ 7(2019):17. |
| Files in This Item: | ||||||
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| peerj-7636.pdf（1739KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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