IMHE OpenIR  > 山地表生过程与生态调控重点实验室
Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China
Bai, Yan1; Huang, Xiying1; Zhou, Xiangrui1; Xiang, Quanju1; Zhao, Ke1; Yu, Xiumei1; Chen, Qiang1; Jiang, Hao2; Nyima, Tashi3; Gao, Xue3; Gu, Yunfu1
Corresponding AuthorGu, Yunfu(guyf@sicau.edu.cn)
2019-08-14
Source PublicationPEERJ
ISSN2167-8359
Volume7Pages:22
AbstractBackground. The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier's retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods. The diversity, community composition, and abundance of nosZ-denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results. nosZ-denitrifiers were more abundant and diverse in soils from successional years 20-60 compared to 0-5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40-60 years) than in younger soils (<= 20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rubrivivax (95 bp) were dominant in soisl during early successional stages (0-20 years) and in the mature phase (40-60 years), respectively. Specific T-RFs of Bradyrhizobium (100 bp) and Pseudomonas (275 bp) were detected only in Site3 and Site4, respectively. Moreover, the unidentified 175 bp T-RFs was detected only in Site3. Of the abiotic factors that were measured in this study, soil available phosphorus, available potassium and denitrifying enzyme activity (DEA) correlated significantly with the community composition of nosZ-denitrifiers (P < 0.05 by Monte Carlo permutation test within RDA analysis).
KeywordHailuogou Glacier retreat area Primary succession Denitrifying bacteria Community Abundance
DOI10.7717/peerj.7356
Indexed BySCI
WOS KeywordNITROUS-OXIDE REDUCTASE ; MICROBIAL COMMUNITY ; SOIL DEVELOPMENT ; GONGGA MOUNTAIN ; NUTRIENT LIMITATION ; NOSZ GENES ; ABUNDANCE ; CHRONOSEQUENCE ; FERTILIZATION ; WATER
Language英语
Funding ProjectScience and Technology Department of the Tibet and Sichuan Agricultural University
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000480819100006
Funding OrganizationScience and Technology Department of the Tibet and Sichuan Agricultural University
PublisherPEERJ INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imde.ac.cn/handle/131551/26967
Collection山地表生过程与生态调控重点实验室
Corresponding AuthorGu, Yunfu
Affiliation1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Sichuan, Peoples R China
2.Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu, Sichuan, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Resources & Environm Sci, Lhasa, Tibet, Peoples R China
Recommended Citation
GB/T 7714
Bai, Yan,Huang, Xiying,Zhou, Xiangrui,et al. Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China[J]. PEERJ,2019,7:22.
APA Bai, Yan.,Huang, Xiying.,Zhou, Xiangrui.,Xiang, Quanju.,Zhao, Ke.,...&Gu, Yunfu.(2019).Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China.PEERJ,7,22.
MLA Bai, Yan,et al."Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China".PEERJ 7(2019):22.
Files in This Item:
File Name/Size DocType Version Access License
peerj-7356.pdf(974KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Bai, Yan]'s Articles
[Huang, Xiying]'s Articles
[Zhou, Xiangrui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Bai, Yan]'s Articles
[Huang, Xiying]'s Articles
[Zhou, Xiangrui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Bai, Yan]'s Articles
[Huang, Xiying]'s Articles
[Zhou, Xiangrui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: peerj-7356.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.